Figure 2

Kindlin-2 subcellular localization. A.-D. Confocal micrographs of immunohistochemistry using anti-kindlin-2 and anti-β1 integrin antibodies on undifferentiated (A; GM) C2C12 cells and C2C12 cells induced to differentiate for 2 (B; DM2) or 4 days (C, D; DM4). Kindlin-2 co-localizes with β1 integrin at sites of cell-cell and cell-matrix contacts (arrowheads in A, B, and D). Kindlin-2 is found at developing costameres at DM2 and DM4 (*). Scale bar = 20 μm. E. Immunostaining of quadriceps muscle from a two month-old mouse. Kindlin-2 localizes to the sarcolemmal membrane and is enriched in the perinuclear compartment (**).