Kinetics of Nrg-GFP fluorescence recovery. Photobleaching in the horizontal plane of epithelial cells from wild-type (A), nrx IV (B) cont (C) and Dfvari (D) embryos. Data are normalized to yield an intensity of zero immediately after photobleaching and represents the average of 7–9 individual traces (mean ± SEM). The traces were obtained using FRAP sequences from multiple embryos [wild-type (n = 3), nrx (n = 5), cont (n = 3) and vari (n = 2)]. Statistical analysis using ANOVA indicates that nrx IV and cont mutations significantly (P < 0.05) increased the mobile fraction of Nrg-GFP as compared with wild-type embryos. The mobile fraction of Nrg-GFP in Dfvari embryos is significantly (P < 0.01) increased by comparison with wild-type, nrx IV and cont embryos.