Kinetics of the fluorescence recovery of Nrx IV-GFP. Photobleaching in the horizontal plane of epithelial cells from wild-type (A), Dfvari (B) and cora (C) embryos. Data are normalized to yield an intensity of zero immediately after photobleaching and represents the average of 9 individual traces (mean ± SEM). The traces were obtained using FRAP sequences from multiple embryos [wild-type (n = 3), cor (N = 4) and vari (N = 2)]. Statistical analysis using ANOVA indicates that the loss of Vari or Cora significantly (P < 0.01) increased the mobile fraction of Nrx IV-GFP as compared with wild-type embryos.