NIH3T3 cells were transfected with indicated constructs. Three days after transfection cells were harvested and a fifth seeded onto glass coverslips. Two hours after seeding cells were fixed. Nuclei number of transfected cells was determined after F-actin and DNA staining respectively with TRITC-coupled phalloidin and DAPI. Total cell number and the number of binucleated cells from a representative experiment are presented as well as the deduced percentage of binucleated cells.