Inhibition of cytokinesis by wiskostatin does not rely on N-WASP/Arp2/3 complex pathway

Table 1 Effect of various N-WASP constructs onto cell ploidy.

Vector	Total cell number	Number of binucleated cells	% of binucleated cells
GFP	419	17	4.06
GFP VCA	527	61	11.57
GFP CA	629	30	4.77
GFP N-WASP	351	19	5.41
GFP N-WASP ΔA	130	7	3.38

NIH3T3 cells were transfected with indicated constructs. Three days after transfection cells were harvested and a fifth seeded onto glass coverslips. Two hours after seeding cells were fixed. Nuclei number of transfected cells was determined after F-actin and DNA staining respectively with TRITC-coupled phalloidin and DAPI. Total cell number and the number of binucleated cells from a representative experiment are presented as well as the deduced percentage of binucleated cells.

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