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Table 1 Effect of various N-WASP constructs onto cell ploidy.

From: Inhibition of cytokinesis by wiskostatin does not rely on N-WASP/Arp2/3 complex pathway

Vector Total cell number Number of binucleated cells % of binucleated cells
GFP 419 17 4.06
GFP VCA 527 61 11.57
GFP CA 629 30 4.77
GFP N-WASP 351 19 5.41
GFP N-WASP ΔA 130 7 3.38
  1. NIH3T3 cells were transfected with indicated constructs. Three days after transfection cells were harvested and a fifth seeded onto glass coverslips. Two hours after seeding cells were fixed. Nuclei number of transfected cells was determined after F-actin and DNA staining respectively with TRITC-coupled phalloidin and DAPI. Total cell number and the number of binucleated cells from a representative experiment are presented as well as the deduced percentage of binucleated cells.