Single cell analysis of siRNA knockdown of GFP. siRNAs transfected at increasing doses into RWPE-1 cells stably transduced to constitutively express GFP, are correlated with the reduction of GFP expression, as determined by fluorescence intensity. A. GFP-siRNA accumulation and correlation with GFP levels observed by fluorescence microscopy. B. Average GFP fluorescence levels of wells treated with a GFP-specific siRNA or a non-targeting control siRNA, as indicated. Each box plot displays the median and intrerquartile range of 8 wells. C. For the transfection of siRNAs at a concentration of 3.13 nM, the cells of one well are plotted individually for both GFP and rhodamine fluorescence intensities.