Figure 2
Overexpression of full-length, wild-type eGFP-myosin-Vb causes coalescence of peripheral endocytic compartments and inhibits perinuclear accumulation of transferrin. HeLa cells were transiently transfected with full-length, wild-type myosin-Vb tagged with eGFP and imaged 24 h after transfection. (A) Diagram depicting predicted results. (B, C) Colocalization of actin on enlarged compartments (arrows) with eGFP-myosin-Vb. (D, E, F) In cells expressing high levels of eGFP-myosin-Vb coincident with exposure to transferrin (arrows), large, peripheral organelles decorated with myosin-Vb also contain transferrin. (G, H, I, J, K, L) In cells exposed to a 1-min pulse of transferrin 24 h after transfection and 10 min before imaging, transfected cells (arrows) contain large, peripheral organelles decorated with myosin-Vb that lack transferrin. Cells expressing lower levels of myosin-Vb (arrowheads, panel K; too low to be seen in panel J) accumulate less transferrin than the surrounding untransfected cells (arrowheads). Bar, 15 μm.