Figure 2

Myriocin and sphingomyelinase treatment decreases [3H]-oleate uptake. COS cells were either grown before cotransfection of FAT/CD36 and FATP4 for 3 d in the presence or absence (control) of 5 μM myriocin or were treated after co-transfection for 15 min with 1 U sphingomyelinase. (A) Overexpressing FAT and FATP4 increased [3H]-oleate uptake significantly (p < 0.05). Myriocin and sphingomyelinase treatment inhibited [3H]-oleate uptake significantly (p < 0.05). Data are expressed as mean and SEM from n = 3 independent experiments. (B) Staining of COS cells with Rh-CTB after 3 d treatment with 5 μM myriocin. The amount of the ganglioside GM1 on the surface is reduced after myriocin treatment. Bar: 200 μm. Both images in panel B were recorded with the same exposure time.