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Figure 3 | BMC Cell Biology

Figure 3

From: Uptake of long chain fatty acids is regulated by dynamic interaction of FAT/CD36 with cholesterol/sphingolipid enriched microdomains (lipid rafts)

Figure 3

Co-patching of PLAP and GFP-TMD (Podo) with FAT/CD36. 20 h after transient transfection, Vero cells were incubated for 45 min at 10°C with respective primary antibodies, washed and incubated for 45 min with mixed Cy5 and Cy3 fluorescently-labelled secondary antibodies. PLAP and GFP-TMD were patched with polyclonal rabbit anti PLAP and polyclonal serum KG77 against GFP, respectively. Patching of FAT/CD36 was achieved with monoclonal antibody from Biosource international. FAT/CD36 co-localized with the raft marker protein PLAP and segregated from GFP-TMD. Bar: 10 μm.

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