Skip to main content
Figure 1 | BMC Cell Biology

Figure 1

From: Focal Adhesion Kinase contributes to insulin-induced actin reorganization into a mesh harboring Glucose transporter-4 in insulin resistant skeletal muscle cells

Figure 1

Effect of insulin on subcellular distribution of FAK and Actin. C2C12 cells were differentiated under insulin sensitive (MF) and insulin resistant (MFI) condition and stimulated with or without insulin for 30 min at 37°C after 4 h starvation followed by fixation and permiabilization and probed with anti-FAK antibody. Cells were subjected to immunofluorescence microscopy by using anti-mouse FITC-conjugated secondary antibody for FAK and Phalloidin Texas Red for actin. Bar corresponds to 5 μm. Images were captured from different fields and a representative image of 3 experiments is presented. a: C2C12 cells differentiated under MF condition, b: C2C12 cells differentiated under MF condition, stimulated with insulin, c: C2C12 cells differentiated under MFI condition, d: C2C12 cells differentiated under MFI condition, stimulated with insulin. White arrow head represent actin filaments, Yellow arrow head represent FAK localization and Blue arrow head indicates colocalized FAK and actin.

Back to article page