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Figure 3 | BMC Cell Biology

Figure 3

From: Focal Adhesion Kinase contributes to insulin-induced actin reorganization into a mesh harboring Glucose transporter-4 in insulin resistant skeletal muscle cells

Figure 3

Effect of FAK expression modulation on insulin-mediated actin remodeling and Glut-4 translocation. Glut-4 transfected cells expressing endogenous level of FAK (C2Glut-4wt/+), overexpressing FAK (C2Glut-4wt/+FAKwt/+) and underexpressing (silenced) FAK (C2Glut-4wt/+FAKwt/-) were differentiated under MF and MFI condition, serum starved (4 h) and stimulated with and without 100 nM insulin for 30 min at 37°C followed by fixation and permeabilization. Actin filaments were labeled with Phalloidin Texas Red, nucleus with DAPI. Images were captured from different fields and a representative image of 3 experiments is presented. Bar represents 5 μm. A, B and C: C2Glut-4wt/+, C2Glut-4wt/+FAKwt/+ and C2Glut-4wt/+FAKwt/- cells differentiated under MF condition; D, E and F: C2Glut-4wt/+, C2Glut-4wt/+FAKwt/+ and C2Glut-4wt/+FAKwt/- cells differentiated under MF condition stimulated with insulin (MF+I); G, H and I: C2Glut-4wt/+, C2Glut-4wt/+FAKwt/+ and C2Glut-4wt/+FAKwt/- cells differentiated under MFI condition; J, K and L: C2Glut-4wt/+, C2Glut-4wt/+FAKwt/+ and C2Glut-4wt/+FAKwt/- cells differentiated under MFI condition stimulated with insulin (MFI+I). White arrow head represent actin filaments, Yellow arrow head represent Glut-4 molecules and Blue arrow head indicates colocalized actin with Glut-4.

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