Figure 4
From: Establishment and characterization of immortalized Gli-null mouse embryonic fibroblast cell lines
Hedgehog pathway specificity in migratory response of Gli2-/-3-/- iMEFs. A. Migration of Gli2-/-3-/- iMEFs was performed using the Smo agonist purmorphamine or 20% FCS as attractants as well as 2 μg/ml Shh in the presence of no inhibitor (control), the Smo antagonist cyclopamine or the LPS inhibitor PMB. Preincubation time with inhibitors following detachment was 10 minutes. B. Migration responses of Gli2-/-3-/- iMEFs transfected with a SuFu or control vector or preincubated with the leukotriene synthesis inhibitor MK-886 or translation inhibitor, cycloheximide. No-attractant condition was subtracted and migration starting points were set to t = 0.