Phosphorylation of p130Cas initiates Rac activation and membrane ruffling

Table 1 Quantification of membrane ruffling in SYF cells following plasmid transfection

Treatment	Percent cells showing membrane ruffling
Mock	21.9 ± 3.65
ΔSrc	31.4 ± 0.96
ZipA ΔSrc	28.7 ± 2.72
ZipB p130Cas	25.6 ± 1.85
ZipB p130Cas + ΔSrc	32.8 ± 2.14
ZipB p130Cas + ZipA ΔSrc	56.9 ± 0.67*

SYF cells were transfected and fixed as described in Materials and Methods and membrane ruffling observed by confocal microscopy (a membrane ruffle is defined as a narrow, actin-rich upward protrusion of the membrane). Data is expressed as percent mean ± S.E.M. (standard error of mean) of three independent observations and was analyzed by one-way ANOVA (analysis of variance), followed by Tukey's multiple comparison test. * denotes p < 0.001 as compared to ZipA ΔSrc transfected group or ZipB p130Cas transfected group. ΔSrc = v-Src lacking SH2 and SH3 domains, ZipA ΔSrc = ΔSrc containing a coiled-coiled domain (FIT-compatible Src), ZipB p130Cas = p130Cas containing a complimentary coiled-coiled domain (FIT-compatible p130Cas).

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