Figure 5

Ik2 does not promote Spn-F degradation. (A) Western blot analysis for Spn-F protein levels. S2 cells were co-transfected with GFP-Spn-F and increasing amounts of GFP-Ik2 (0, 200, 500,1000 ng). Ik2 did not affect Spn-F protein levels. (B) Western blot analysis for Spn-F protein stability in the germline. The level of Spn-F in the germline was examined in the presence of over-expression of Ik2. Ovarian extracts from flies expressing GFP::Spn-F and GFP::Ik2 was tested in comparison with ovarian extracts from transgenic flies expressing GFP::Spn-F or GFP. Our results showed that expression of Ik2 does not affect Spn-F stability in the germline.