Figure 1

Localization of p36 to the outer mitochondrial membrane in BY-2 cells. Tobacco BY-2 cells were either non-transformed or transformed transiently (via biolistic bombardment) with either myc-p36 or p36-myc and then processed for immunofluorescence CLSM. Hatched boxes represent the portion of the cells shown at higher magnification in the panels to the right. Arrowheads indicate obvious examples of the torus fluorescent structures containing myc-p36 or p36-myc either delineating the spherical structures containing endogenous mitochondrial matrix-localized E1β or colocalizing with the torus structures containing the endogenous mitochondrial outer membrane protein porin. The bottom row of images show representative immunofluorescence staining patterns attributable to endogenous porin and E1β in non-transformed cells; arrowheads in the high magnified images indicate obvious examples of porin-containing torus structures (similar to those containing myc-p36 or p36-myc [compare with cells above]) enclosing the E1β-containing spheres. Bar = 10 μm.