Figure 2

Topological orientation of p36 in differentially permeabilized BY-2 cells. BY-2 cells were either non-transformed or transformed transiently (via biolistic bombardment) with myc-p36 or p36-myc, fixed, and then permeabilized with either triton X-100 (which permeabilizes both the plasma membrane and organellar membranes) or digitonin (which permeabilizes only the plasma membrane). Permeabilized cells were then processed for (immuno)epifluorescence microscopy using antibodies raised against (as indicated by the labelling at the top left of each micrograph) either cytosolic α-tubulin, mitochondrial matrix-localized E1β, the myc epitope and/or the p36 C-terminal peptide sequence (amino acids 218–237) located downstream of the protein's second (of two) predicted TMDs. Bar = 10 μm.