Figure 2

Cdc11 localizes to tubulin cytoskeleton during meiosis before its association with the prospore membrane. (A). Sporulating wild-type cells were stained with anti-Cdc11 and anti-tubulin antibodies and DAPI. Cells in different phases of meiosis are shown: (1), prophase; (2), metaphase I; (3) anaphase I; (4), metaphase II; (5), anaphase II; (6–7), post-meiotic cells. (B) Confocal microscopy of wild-type cells stained with anti-Cdc11 and anti-tubulin antibodies. The merged image shows Cdc11 (green) and tubulin (magenta) and white indicates areas where the two proteins colocalize. Cells in prophase (1) or anaphase II (2) are shown. (C) Cdc11 and Tub1 co-immunoprecipitate during sporulation. Protein extracts prepared from sporulating cells carrying TUB1-GFP (sporulation) or grown to mid-logarithmic phase (vegetative) were incubated in the presence (lane 3) or absence (lane 1) of anti-Cdc11 antibody. Extracts from the wild-type strain grown in the same conditions were used as controls (lane 2). Proteins were probed with anti-GFP antibody. The lower panels show a Western blot of the extracts used for the immunoprecipitations.