Figure 7
From: Septins localize to microtubules during nutritional limitation in Saccharomyces cerevisiae
Non-fermentable carbon sources induce Cdc11 localization to microtubules. (A) AN117-16D haploid cells were inoculated in media containing glucose (YPD), acetate (YPA) or glycerol (YPG) as carbon sources or in media with poor nitrogen sources (SPD or MM-I). For MM-II medium, AN999 (MATα, lys1) was used. Cells were stained with anti-Cdc11 antibodies. The percentage of cells with bud neck (white), bud neck and microtubules (squares), microtubules (grey) or diffuse cytoplasmic Cdc11 staining (black) is shown in the graphs. Representative cells are also shown. (B) Cdc11 distribution in AN117-16D haploid cells or the rho- derivative grown in YPA. (C) AN117-16D haploid cells were grown in YPA for 16h and then transferred to AcK 2% or AcK 2% containing 15 mM sodium azide. The percentage of cells with different Cdc11 localizations 4 h after the inoculation is shown in the graph.