Figure 5
Dual-localization of co-expressed β 2 -adrenergic receptors by confocal microscopy. HEK293 cells stably expressing Flag-tagged β2AR-Gαs receptor were transiently transfected with a HA-tagged β2AR construct to obtain the co-expression of the two receptors in the same cell. Cells were treated (Agonist) or not (Control) with 1 μM isoproterenol for 5 hours, fixed and subjected to indirect immunofluorescence analysis as described in Methods, and then visualized by confocal microscopy. Co-localization of HA-β2AR (Alexa-Fluor 488 staining; green) with Flag-β2AR-Gαs (Alexa-Fluor 594 staining; red) is shown in the merged colour image by yellow staining. Photographs represent a typical situation observed in 4 independent experiments. Scale bars, 10 μm.