Figure 4
From: Aggresomes do not represent a general cellular response to protein misfolding in mammalian cells
Oligomeric DsRed2 does not fluoresce when genetically fused to CyPrP. (A) Direct fluorescence or immunofluorescence (3F4 anti-PrP antibodies, green) of N2a and Hela cells expressing CyPrPDsRed2. Nuclei were stained with Hoechst (blue). Blue and red channels are shown merged. (B, C) Direct fluorescence of N2a and Hela cells expressing CyPrP124stopDsRed2 (B) or CyPrPmOrange (C). Nuclei were stained with Hoechst (blue). Blue and red channels are shown merged.