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Figure 4 | BMC Cell Biology

Figure 4

From: Phosphatidylinositol 3-kinase signaling in proliferating cells maintains an anti-apoptotic transcriptional program mediated by inhibition of FOXO and non-canonical activation of NFκB transcription factors

Figure 4

Analysis of FOXO binding sites by chromatin immunoprecipitation. T98G cells were transfected with an empty pcDNA3 control or Flag-FOXO3a-AAA expression vector for 24 hours. Chromatin fragments were immunoprecipitated with anti-Flag antibody and quantified by real-time PCR (see Additional file 4 for primers). Data are presented as percentage of input and are the means of 3 independent transfections ± S.E β-globin is a negative control. Immunoblots indicated that the expression of FOXO3a-AAA in transfected cells was 3–5 fold greater than the endogenous protein.

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BMC Molecular and Cell Biology

ISSN: 2661-8850

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