Phosphatidylinositol 3-kinase signaling in proliferating cells maintains an anti-apoptotic transcriptional program mediated by inhibition of FOXO and non-canonical activation of NFκB transcription factors

Table 5 FOXO binding sites confirmed by chromatin immunoprecipitation.

Gene	Binding site position	TRANSFAC 8.4	TRANSFAC 11.1	Primer Position
ATROGIN-1	-31		V$HFH8_01	-160
	-118	V$FOXO1_01	V$HFH1_01
	-136	V$FOXO1_01	V$FOXO1_01
BCL6	-837		V$HFH1_01	-1126
	-881		V$FOXO1_02
	-991	V$FOXJ2_01	V$HFH1_01
	-1419		V$HFH1_01
BTG1	-213	V$FOXJ2_01	V$FOXO1_01	-254
	-220		V$HFH1_01
	-496	V$FOXO1_01	V$HFH1_01
CCNG2	-158	V$FOXO1_01	V$HFH1_01	-243
	-165		V$FOXO1_01
	-469		V$FOXO1_01
	-473	V$FOXO1_01	V$FOXO3_01
	-483		V$FOXO1_01
DDIT3/CHOP	-1544		V$FOXO1_01	-1973
	-1606		V$HFH1_01
	-1830		V$FOXO1_02
	-2069		V$FOXO1_01
K1F1B	-673		V$FOXO1_01	-673
KLF6	-955	V$FOXO1_01	V$HFH1_01	-947
	-929	V$FOXO3_01
	-887	V$FOXJ2_01	V$HFH1_01
KLHL24	-14		V$FOXO3_01	-211
	-34		V$FOXO1_01
TXNIP	-216	V$FOXO1_01	V$HFH1_01	-250
ZBTB1	-252		V$FOXO1_02	-263

The predicted FOXO binding sites that were confirmed by ChIP are indicated by the most 5' nucleotide. When multiple matrices predicted overlapping sites, the position indicates the most 5' nucleotide of the consensus sequence (see Additional file 4 for all sequences). The TRANSFAC matrices shown are those that predicted the indicated sites with the lowest p-values. ChIP assays were considered to confirm binding sites located within 500 bp of the primer position. Primer positions are indicated by the most 5' nucleotide of the forward primer; all amplicons were 50–60 bp.

ISSN: 2661-8850