Figure 6

PKC inhibition reverts the effect on the redistribution of Claudin-1 and the paracellular permeability caused by PGE ₂ . The TER was measured before and after treatment with 1 μM PGE₂ and pretreated for 1 h with H-89, SB203580 and Calphostin C. Note that the decrease of the TER was PKC-dependent and H-89 abrogated the TER recovery at 60 and 120 min (A). Caco-2 cells were grown until confluence treated or pretreated with Calphostin C, prior to incubation with 1 μM PGE₂and the redistribution of Claudin-1 was assessed by immunoblotting (B) and immunofluorescence (C). The effect of pretreatment with the inhibitor was also analyzed by using the ruthenium red technique and electron microscopy (D). Observe that PKC inhibition was able to block alterations caused by PGE₂. Bars in B: 10 μm and in D: 1.2 μm.