Cell adhesion on C100 Agrin nanopatterned substrates. Mouse primary cortical neurons (A) showed significantly higher adhesion to 30 nm and 60 nm spaced substrates than to 90 nm and 160 nm spaced substrates. Adhesion to more closely spaced substrates is comparable to homogenously coated agrin (first bar). Rat B35 neuroblastoma cells (B) showed a similar behaviour. Cell Adhesion on N-Cadherin nanopatterned substrates. Primary mouse cortical neurons (C) and Rat B35 neuroblastoma cells (D) do not show a significant decrease in cell adhesion with increasing spacing of the nanopatterned substrates, and adhesion is always below the level observed for uniformly coated N-Cadherin (first bar). This suggests a different mechanism for N-Cadherin mediated cell adhesion than for Agrin mediated cell adhesion.