Arp2/3 complex localisation to filopodia is independent of adhesion. A. Filopodia of MEF spread on 5 μm fibronectin stripes for 60 minutes. Arp2/3 complex is present on BSA stripes. Blue, Cy3 fibronectin, green, Arp2/3 complex (antibody to p34-Arc sub-unit) and red (rhodamine phalloidin), actin. Left and middle panel scale bar 10 μm, right panel 5 μm. B. MEF spread on 10 μm fibronectin stripes for 60 minutes. Scale bar 10 μm. C. Enlargement and deconvolution of boxed area in B. Scale bar 10 μm.