Figure 5
Effects of β-catenin knockdown on Ras-ERK pathway activation and VPA-induced differentiation and inhibition of proliferation. NPCs were transfected with 100 nM control siRNA or β-catenin siRNA prior to treatment with 1 mM VPA for 48 h. (A) Whole-cell lysates were subjected to immunoblotting to detect presence of β-catenin, Pan-Ras, p-ERK, p21Cip/WAF1, Tuj1, EGFR, or β-actin. (B-C) Immunofluorescent labeling of Tuj1or BrdU. Nuclei were counterstained with DAPI.