CK18 RNAi treatment does not inhibit trypomastigote attachment or invasion of HeLa cells. HeLa cells were left untreated or treated with CK18 RNAi or control RNAi. After 24 h, cells were infected with culture trypomastigotes of the Y strain of T. cruzi. (A) Number of trypomastigotes attached to HeLa cells; (B) Frequency of infected HeLa cells; (C) Number of parasites per 100 cells. Bars represent the average of triplicate cultures ± SD.