CK18 RNAi treatment does not inhibit lysosome recruitment to the parasitophorous vacuole and parasite escape to cytoplasm. HeLa cells were left untreated or treated with CK18 RNAi or control RNAi. After 24 h, cells were infected with culture trypomastigotes of the Y strain of T. cruzi. The frequency of parasites stained with anti-LAMP-1 MAb is shown at the indicated hours post-infection. Dots represent the average of triplicate cultures ± SD.