Ebp1 is not expressed in Ebp1-/- animals. A, B. Fibroblasts were derived from 12.5 day Ebp1-/- (KO) and wild type (WT) embryos as described in the Materials and Methods. A. RNA was isolated and the expression of Ebp1 and actin was determined by conventional PCR analysis. B. The expression of Ebp1 in KO and WT MEFs was analyzed by Western blot analysis. C. The expression of Ebp1 from lung (L) and kidneys (K) of 10 week old Ebp1-/- and wild type littermates was detected by Western blot analysis.