Validation of differential Cyclin D1 expression in wild type and Ebp1 knock out MEFs. A. Conventional PCR analysis of Cyclin D1 and actin mRNA in wild type and knock out MEFs. B. Real Time quantitative RT-PCR analysis of Cyclin D1 mRNA. The relative level of Cyclin D1 was normalized to β actin. Results are representative of 2 experiments using different sets of cells. C. Expression of Cyclin D1 protein in wild type and knock out MEFs. Lysates of Ebp1 knock out or wild type MEFs were resolved by SDS-PAGE and analyzed by western blotting with the indicated antibodies.