Changes in expression of Ebp1 target genes in adult tissue. A. Number 4 mammary glands were isolated from 10 week old wild type (WT) or Ebp1 knock out (KO) mice. Immunohistochemical analysis was performed for phospho AKT (p-AKT) or total AKT (T-AKT) as indicated. B. Expression of AR and AR regulated genes in prostates. The expression of AGR2 in the anterior prostates of age matched wild type and Ebp1 knock out mice were measured by real-time PCR (left panel) or Western blot (middle panel) analysis. AR levels were measured in lysates of anterior prostates by Western blot analysis (Right panel).