Alterations of insulin signaling pathways in adult Ebp1 knock out mice. A. Serum IGF-1 as measured by ELISA. IGF-1 levels in serum from Ebp1 knock out and wild type mice were assessed by ELISA as described in the Materials and Methods. B. Liver and kidneys of 3 month old wild type (WT) and knock out (KO) mice were analyzed by Western blotting for expression of IGFBP-3 or actin as indicated. Blots were analyzed by densitometry using Image-J software. Results presented underneath each lane are the ratios of pixel densities of IGFBP-3 to actin bands.