Figure 3

C heiradone inhibited cell migration. (A) A cell monolayer was wounded as described and recovery was assessed in the presence of increasing concentration of cheiradone (0–7.7 μM) and VEGF₁₆₅ (10 ng/ml in all cases). Controls without VEGF contained either DMSO or cheiradone (7.7 μM). Values significantly different from VEGF alone (p < 0.05) are shown by *. (B) Representative photomicrographs show the results for HDMEC: i control, DMSO alone; ii time zero control; iii cheiradone 7.7 μM; iv treated with VEGF₁₆₅; 10 ng/ml, v cheiradone + VEGF. (C) The effect cheiradone on VEGF-induced cell migration in a three dimensional Boyden chamber assay. Cells were added to the upper chamber and the cheiradone (0–7.7 μM) with and without VEGF (10 ng/ml) were added to the lower part. The total number of migrated cells to the lower chamber was counted as described above. Controls without VEGF contained either DMSO or cheiradone (5 μM). Values significantly different from VEGF alone (p < 0.05) are shown by *.