Figure 6

Localization of AmtC in vegetative cells. (A) Perinuclear localization of AmtC-YFP. Compound images of AmtC-YFP (left), Hoechst stained nuclei (middle) and merged (right). Cells were fixed briefly in 70% ethanol and washed with DB prior to and after staining with Hoechst. Several cells are shown. (B) Plasma membrane localization of AmtC-RFP. Compound images of AmtC-RFP (left), FITC-dextran loaded endosomes (middle) and merged (right). (C) AmtC-RFP localization on the plasma membrane and some internal organelle membranes when co-expressed with AmtA-YFP in growing cells. (D) Localization of AmtC-RFP on phagosome membranes when co-expressed with AmtA-YFP. Cells were incubated with live yeast (arrowheads) to induce phagocytosis. (E) Apparent complete colocalization of AmtC-RFP with AmtB-YFP in growing cells. (F) Localization of AmtC-RFP on phagosome membranes when co-expressed with AmtB-YFP. Cells were incubated with live yeast to induce phagocytosis. Images C-F are confocal with 5 μm scale bars.