Figure 4

Intracellular localization of p49 protein in cultured H9C2 cells. Image analyses was performed on more than 200 H9C2 cells transfected with p49-GFP at 1,2 and 3 days after transfection. Cytosolic and nuclear signal intensity of p49 was computed using "Image J" program (see description in "Methods"). Top Panel: No significant relationship was found between p49-GFP cytosolic fraction and day post-transfection(Day, x axis). Bottom Panel: Images of cells with p49-GFP evident in the extranuclear region were measured after subtracting the background noise. The images were grouped based on the number of days post-transfection. In our studies, cell growth displayed a negative quadratic function during days 1–3 post-transfection (R2adj = 0.273, regression not shown).