Figure 6

Co-expression of p49 and NDUFAB1. Fluorescent images of H9C2 cells showing uneven distribution p49-EGFP fusion protein alone, in green (a) and NDUFAB1-RFP fusion protein alone, in red (b). Co-expression of p49 and NDUFAB1 fusion proteins indicates co-localization of these two proteins that appear as yellow in the nuclear region (c). These panels, taken together with those of figs. 3a-3d, suggest a more focal localization of p49-GFP compared to control (a, c) in these cells. However, for the NDUFAB1-RFP construct (b, c), the distribution usually appears to be more even throughout the cell, similar to that of GFP-only and RFP-only controls (d, e, f). GFP = green fluorescent protein; RFP = red fluorescent protein.