Figure 8

p49/STRAP reduces the level of acetylated-SRF protein. H9C2 cells were transfected with p49 plasmid construct (p49-plasmid) or with empty vector (empty vector) and harvested after 24 hours. H9C2 cells without transfection (No treatment) were used as controls. Equal amounts of the protein from the above samples were used to immunoprecipitate the SRF protein, using an antibody recognizing the C-terminal SRF protein (Santa Cruz). The immunoprecipitated SRF proteins were then subjected to SDS-PAGE and Western blotting. The level of total SRF protein in the samples was determined using the same anti-SRF antibody. The acetylated-SRF was detected by an antibody specifically recognizing the acetyl-lysine (clone 4G12, Upstate biotechnology). The results indicate that p49/STRAP overexpression reduces the level of acetylated-SRF protein.