Identification of a subunit of NADH-dehydrogenase as a p49/STRAP-binding protein

Table 1 The NDUFAB1 protein selectively binds to the N-terminus of p49 protein (p49-N).

Origin of cDNA Library	Screening bait	Clones positive for NDUFAB1	Main target
Haert	p49-N	95%	NDUFAB1
Hela	p49-N	40%	NDUFAB1
Hela	p49-C	0%	Other

Three separate yeast two-hybrid screenings were performed using p49-N and p49-C as bait, respectively. When p49-N was used to screen human heart cDNA library, 42 out of 44 (95%) of the positive clones that interacted with p49-N were NDUFAB1. When p49-N was used to screen Hela cell cDNA library, 8 out of 20 (40%) of the positive clones were NDUFAB1. However, when p49-C was used to screen Hela cell cDNA library, none of the clones was NDUFAB1. These results indicate that p49-N selectively binds to NDUFAB1.

ISSN: 2661-8850