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Figure 4 | BMC Cell Biology

Figure 4

From: Identification of a novel Leucine-rich repeat protein and candidate PP1 regulatory subunit expressed in developing spermatids

Figure 4

TLRR associates tightly with the KIFC1 targeting sequence. (A) 200 μg testis lysate (lane 1 and 2) or an aliquot of recombinant HIS6 tagged TLRR (lane 3) were resolved by SDS-PAGE and blotted with TLRR antibody (lane 1 and 3) or preimmune serum (lane 2). (B) Testis lysate was loaded onto a column consisting of KIFC1 targeting peptide-linked resin. Flow through (FT) was collected, the column washed and bound complexes eluted with glycine, pH 2.5 (lane 1) or glycine plus 0.5 M NaCl (lane 2). Coomassie stained gel is shown at top with TLRR western below.

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BMC Molecular and Cell Biology

ISSN: 2661-8850

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