TLRR localizes near the distal pole of the spermatid nucleus. (A-B) Merged images of adult mouse testis sections triple stained with anti-TLRR (red), peanut agglutinin (green, acrosome) and DAPI (blue, nuclei). (A'-B') TLRR signal corresponding to each of the merged images shown in A-B. TLRR is found near the nuclear membrane at a site opposite the acrosome and is most prominent in later step spermatids (arrowheads, panels B and B'). (C, C') Control staining for this series where TLRR antibody was replaced with normal rabbit IgG. Bar represents 10 μm.