Rab27a and Rab27b promote neutrophil chemotaxis in vitro . (A) Immunoblot analysis of Rab27a and Rab27b expression using 50 μg lysates from bone marrow derived neutrophils (BMDN) purified from wild-type, Rab27b KO and Rab27DKO mice. Antibody recognized both Rab27a (upper band) and Rab27b (lower band). Transwell migration of BMDN from wild-type, Rab27b KO and Rab27DKO across a range of concentrations of (B) MIP-2 and (C) LTB4 after 30 min. (D) Comparison of transwell migration of wild-type, ashen, Rab27b KO and Rab27DKO BMDN using MIP-2 after 30 min. * p ≤0.05, *** p ≤0.001 using ANOVA. Error bars s.e.m. Data representative of 3 similar experiments.