Figure 3
From: Isolation and epithelial co-culture of mouse renal peritubular endothelial cells
Immunofluorescence staining assessment of MicroBeads isolated peritubular endothelial cells after 2 days culture. Immunofluorescence staining of isolated peritubular endothelial cells against VE-cadherin (green, A), FSP-1 (green, indicated by white arrow, B), CD11c (green, C), F4/80 (green, E), and positive control for CD11c (primary spleen dendritic cells, green, D) and F4/80 (primary spleen macrophage, green, F), Immunofluorescence staining against pericyte marker CD73 (green, G) and PDGFR-β (green, I), and positive control for CD73 (frozen kidney sections of BALB/c mouse, green, H) and PDGFR-β (frozen kidney sections of BALB/c mouse, green, J). (K) Statistical analysis of each individual marker immunofluorescent stained and quantified as a percentage of positively stained cells against total cells (2000). Data are expressed as mean ± SEM with N = 10 for each experimental group. Original magnification was x 200; original magnification for pericytes staining was x 400. Cells in this figure were counterstained with DAPI to visualize nuclei (blue).