Table 1 Components of collagenase type IV solution
From: Isolation and epithelial co-culture of mouse renal peritubular endothelial cells
Collagenase D solution | Volume or concentration | Supplier |
|---|---|---|
DMEM/F12 medium | 7.5 ml | Gibco Life Technologies; Grand Island, NY, USA |
Collagenase type IV | 1 mg/ml | Sigma Chemical Co.; St Louis, MO, USA |
Deoxyribonuclease | 0.1 mg/ml | Sigma Chemical Co. |
Bovine serum albumin (BSA) | 1 mg/ml | Sigma Chemical Co. |