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Table 2 Percoll solution (25 ml)

From: Isolation and epithelial co-culture of mouse renal peritubular endothelial cells

Percoll solution

Volume

Supplier

DMEM/F12 medium

12.5 ml

Gibco Life Technologies; Grand Island, NY, USA

Percoll (45%, vol./vol.)

11.25 ml

Gibco Life Technologies

20 X concentrated PBS

0.5625 ml

AMRESCO; Solon, OH, USA

MilliQ water

0.5625 ml

Roche Applied Science; Penzberg, Upper Bavaria, Germany

1 M HEPES

80.5 μl

Gibco Life Technologies