From: Isolation and epithelial co-culture of mouse renal peritubular endothelial cells
Percoll solution | Volume | Supplier |
---|---|---|
DMEM/F12 medium | 12.5 ml | Gibco Life Technologies; Grand Island, NY, USA |
Percoll (45%, vol./vol.) | 11.25 ml | Gibco Life Technologies |
20 X concentrated PBS | 0.5625 ml | AMRESCO; Solon, OH, USA |
MilliQ water | 0.5625 ml | Roche Applied Science; Penzberg, Upper Bavaria, Germany |
1 M HEPES | 80.5 μl | Gibco Life Technologies |