Isolation and epithelial co-culture of mouse renal peritubular endothelial cells

Table 3 Components of hormone mixture in K1 medium

Ingredient	Supplier	Stock solution	Vol./wt. of addition
Insulin	Sigma Chemical Co., St. Louis, MO, USA	Powder	50 mg dissolved in 10 ml of HBSS/HEPES* and small amount of NaOH
Prostaglandin E₁	Cayman Chemical, Ann Arbor, MI, USA	0.5 mg/ml (in EtOH)	25 μl
3,3,5-triiodothyro–nine	Sigma Chemical Co.	16.9 mg/ml (0.026 M) (in EtOH)	2 μl of stock added to 10 ml HBSS/HEPES* then used in 100 μl aliquots
Transferrin	Sigma Chemical Co.	Powder	50 mg
Sodium selenite	Sigma Chemical Co.	0.173 mg/ml (10^-6 M) in HBSS/HEPES	100 μl
Hydrocortisone	Sigma	0.18 mg/ml (in EtOH)	1 ml

The hormone mixture was brought to a final volume of 100 ml with HBSS/HEPES, and aliquoted into 5 ml or 10 ml portions and stored at –80°C.
*HBSS/HEPES = Hanks Balanced Salt Solution without calcium and without magnesium (Gibco, Life Technologies; Grand Island, NY, USA)/1% HEPES.

ISSN: 2661-8850