Figure 1

Human endothelial cells readily differentiate on thin layers of basement matrix spread on glass. (A) Representative images of EA.hy926 and HUVEC forming tube-like structures after 24 and 16 hours, respectively, when plated onto 10 μl/2 cm² of basement matrix in the presence of VEGF (25 ng/ml) or GW0742 (1 μM). Images were acquired using Leica DMIRB microscope (x10 objective). Scale bar = 200 μM. (B) Quantification of tubes formed by HUVEC at 16 hours in the presence of VEGF, GW0742 or DMSO control. Data represent mean (± S.E.M) number of branches/field from n = 5 separate donors. **p < 0.01 vs. DMSO control as determined by repeated measures ANOVA followed by Dunnett's post analysis.