Figure 2From: A thin layer angiogenesis assay: a modified basement matrix assay for assessment of endothelial cell differentiationHuman endothelial cells readily differentiate on thin layers of basement matrix spread on tissue culture plastic. (A) Representative images of HUVEC forming tube-like structures after 16 hours when plated onto 10 μl/2 cm2 of basement matrix in the presence of VEGF (25 ng/ml) or medium only control. Images were acquired using Leica DMIRB microscope (x10 objective). Scale bar = 200 μM. (B) Quantification of branches formed by HUVEC at 16 hours in the presence of VEGF, or medium control. Data represent mean (± S.E.M) number of branches/field from n = 4 separate donors. *p < 0.05 vs. medium control as determined by paired Student's t-test.Back to article page