The thin-layer angiogenesis assay (TLA) can be performed in a 96-well plate format using 2 μl of basement matrix. (A) Representative images showing HUVEC (25,000 cells/cm2) forming tube-like structures after 16 hours when plated onto 2 μl of basement matrix with more tubes forming in the presence of VEGF (25 ng/ml) or GW0742 (100nM). Images were acquired using Leica DMIRB microscope (x10 objective). Scale bar =200 μM. (B) Mean number of branches (± S.E.M)/field formed at 16 hours in the presence of VEGF (25 ng/ml), GW0742 (100nM) or DMSO control. *p < 0.05 vs. DMSO control as determined by repeated measures ANOVA followed by Dunnett's post analysis, n = 6 separate donors ran in sextuplet.