Figure 4

Comparison of manual versus automated quantitation of angiogenic parameters. A comparison of (A) average number of branches/field and (B) average number of junctions/field obtained from the 24-well data acquired from the HUVEC TLA experiments reported in Figure 1 when quantified manually or with the use of the angiogenesis macro for ImageJ. n = 5 independent donors. *p < 0.05 **p < 0.01 ***p < 0.001 vs control as determined by one-way ANOVA followed by Dunnett's post-analysis test.