Up-regulation of PKCδ during osteogenic differentiation in hBMSCs. (A-D) Post-confluent hBMSCs were cultured in GM or ODM for the indicated times. The osteogenic differentiation potential of hBMSCs was estimated by ALP activity assay (A) and RT-PCR analysis of early and late osteogenic markers: ALP and OC (B) at the indicated times. (C) The mRNA expression of PKCδ and δ were determined by RT-PCR at the indicated times after osteogenic induction of hBMSCs. (D) The protein level of PKCδ was determined by western blot analysis with the specified antibodies at the indicated times after osteogenic induction of hBMSCs. Data shown are means ± S.E. (*P <0.05 versus GM) of three independent experiments.