Suppression of PKCδ-mediated osteogenic differentiation enhances the adipogenic phenotype of hBMSCs. Post-confluent hBMSCs were cultured in ODM with 2 μM rottlerin (A) or 10 μM compound C (G). (D) Control shRNA- or PKCδ shRNA-transduced cells were cultured in ODM. Differentiated adipocytes were stained with oil red O after 14 days of osteogenic induction and at magnification of x 100 photographed. (B, E, and H) After 14 days of osteogenic induction, RT-PCR was performed to estimate the mRNA expression of adipocyte-specific genes, including PPARγ, C/EBPα, and aP2, in the indicated groups. (C, F, and I) After 14 days of osteogenic induction, the protein expression levels of PPARγ and C/EBPα were determined by western blot analysis with the specified antibodies in the indicated groups. Data shown are representatives of three independent experiments.